Angiogenesis Assay
| Angiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of a number of normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth and metastasis). Endothelial cells, which are cultured on a solid gel of basement proteins, align and form tube-like structures. This tube formation is a multi-step process involving cell adhesion, migration, differentiation and growth. |
Measurement of the development of tube-like structures is a specific test for angiogenesis. Changes in this process caused by inhibitors of angiogenesis can be determined. An XTT test with HUVEC cells is carried out in parallel to evaluate the cytotoxic potential of a test compound at the same test concentrations. |
Invasion Assay
| Cell migration, the movement of cells from one area to another generally in response to a chemical signal, is central to achieving functions such as wound repair, cell differentiation, embryonic development and the metastasis of tumors. This assay allows the identification of compounds inhibiting migration of tumor cells. 96-well plates with a permeable support insert (8.0 µm PET microporous membrane) are used to evaluate the migratory potential of tumor cells in response to a chemoattractant. |
The amount of cells that have migrated is determined by staining with a fluorescence dye. Changes in the migratory behaviour caused by inhibitors can be determined. An XTT test with the same tumor cell lines is carried out in parallel to evaluate the cytotoxic potential of a test compound at the same test concentrations. |
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