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ONCOTEST offers combination testing of substances in the monolayer assay.
Concept of drug combination testing:
- Analysis of viable cell number by PI assay
- Initial investigation of single drug application for IC50 determination
- Selection of combi layout 1,2 and/or 3) Combinations (A+B), as well as drug A and B alone are represented on one single microtiterplate
- Application of compounds by liquid handling robotic system
- Evaluation of synergism by
- Combination Index (Chou-Talalay)
- Visual inspection of concentration-effect curves
To download information about the combination testing plate layout, please visit our download area. |
Three setups for combination analysis:
- A. Combination at fixed ratio
2 cytotoxic compounds at an equipotent ratio (e.g. at their IC50 values) at 10 concentrations
Determination of Combination Index (CI) according Chou-Talalay by using CalcuSyn Software
Evaluating synergism:
CI<1 indicates synergism
CI=1 indicates additive effect
CI>1 indicates antagonism
- B. Combination at fixed concentration
cytotoxic compound A at 10 concentrations
compound B at non / weak cytotoxic concentration(s)
Evaluation by visual inspection of concentration-effect curve / shift of IC50/IC70 value
- C. Full combinatorial plate layout
2 cytotoxic compounds are tested in a matrix of 7 concentrations
diagonals on a test plate represent equipotent ratio combinations
coulums / rows represent fixed concentration combination
Evaluation by
(i) Combination Index according Chou-Talalay
(ii) visual inspection of concentration-effect curves |
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