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Background:
If the targets of your compounds are known, choosing tumors for further testing based on the targets expression level is the most suitable way. Principle:
The analysis of target expression can either be done on protein or mRNA level. | | | Using Affymetrix Gene Chip Analysis it is possible to measure the expression of every gene in the human genome. This data is already available for more than 180 of Oncotests human tumor xenografts.
|  | Expression analysis of native proteins in tumor lysates can be carried out using bead coupled antibodies detected by flow cytometry (Bead Suspension Assay).
|  | The tissue micro arrays and cell line micro arrays allow the simultaneous analyses of a large collection of samples under the same conditions (Tissue Micro Array).
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Once tumor xenografts have been selected based on target expression, your compounds can be tested for antitumor activity in these xenografts the clonogenic assay and further on in in vivo studies. |
